摘要：
Abstract The extraction of polyphenols from Aronia melanocarpa was carried out using a combination of enzymatic and ultrasound. After single-factor and orthogonal design and experiment, the optimized polyphenol extraction conditions were 1% enzyme, 1:40 material-to-liquid ratio, 55 ℃, 60 min ultrasonication, 70% ethanol, and the fi nal extraction amount was 88.634 mg/g, which displayed a 25.15% and 34.08% improvement compared with the single ultrasonication and enzymatic extraction methods, respectively. Significant antibacterial effects of polyphenols were shown against Staphylococcus aureus, Escherichia coli and Bacillus subtilis. Further antioxidation eff ects were evaluated, and the superoxide anion radical scavenging rate, hydroxyl radical scavenging rate and DPPH free radical scavenging rate reached 45.2%, 83.5% and 85.4%, respectively. This combined enzymatic and ultrasonic extraction method exhibited the advantages of high extraction rate, saving solvent consumption and extraction time, but also provided a new method for the development and utilization of natural antimicrobial and antioxidant health products.
Xinzhu Zhang, Ming Song, Weizhuo Xu, Wei Xu.
Polyphenols extracted from Aronia melanocarpa by combined enzymatic and ultrasonication and their antioxidant potential and antimicrobial activity
[J]. 多酚, 2023, 5(1): 35-47.
Xinzhu Zhang, Ming Song, Weizhuo Xu, Wei Xu. Polyphenols extracted from Aronia melanocarpa by combined enzymatic and ultrasonication and their antioxidant potential and antimicrobial activity[J]. Journal of Polyphenols, 2023, 5(1): 35-47.
