多酚 ›› 2023, Vol. 5 ›› Issue (2): 51-59.

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Optimization of extraction of polyphenols from pomegranate peel by orthogonal experiment design and antioxidant activity

  

  • 出版日期:2023-12-20 发布日期:2023-12-19

Optimization of extraction of polyphenols from pomegranate peel by orthogonal experiment design and antioxidant activity

  • Online:2023-12-20 Published:2023-12-19

摘要: The aim of this study was to investigate the extraction process and antioxidant activity of polyphenols from Yunnan Mengzi pomegranate peel. Single factor and orthogonal test were used as optimize the extract rate (ER) of polyphenols from pomegranate peel (PGP). The polyphenols antioxidant activity was evaluated by DPPH·, ·OH and total reducing ability, and compared with that of Vc. The optimum extraction conditions of polyphenols were as follows: ethanol concentration of 50%, liquid-solid ratio of 50:1, extraction temperature of 90 °C, extraction for 4 h. Under these conditions, the ER of polyphenols from GPG was 8.15%. The results showed that the scavenging eff ects of polyphenols and Vc on DPPH·, ·OH increased with increasing concentration, and the scavenging eff ect of polyphenols on DPPH· was higher than Vc in rang of 0.1-1.6 μg/mL. When the concentration is greater than 0.6 μg/mL, the scavenging eff ects of plyphenols on ·OH is higher than Vc. The total reducing ability of polyphenols from PGP improved with the increase of concentration, and when the concentration was 8 μg/mL, the total reducing ability was 114 U/mL.

关键词: pomeranate peel, total polyphenols, extraction process, orthogonal experiment, antioxidant activity

Abstract: The aim of this study was to investigate the extraction process and antioxidant activity of polyphenols from Yunnan Mengzi pomegranate peel. Single factor and orthogonal test were used as optimize the extract rate (ER) of polyphenols from pomegranate peel (PGP). The polyphenols antioxidant activity was evaluated by DPPH·, ·OH and total reducing ability, and compared with that of Vc. The optimum extraction conditions of polyphenols were as follows: ethanol concentration of 50%, liquid-solid ratio of 50:1, extraction temperature of 90 °C, extraction for 4 h. Under these conditions, the ER of polyphenols from GPG was 8.15%. The results showed that the scavenging eff ects of polyphenols and Vc on DPPH·, ·OH increased with increasing concentration, and the scavenging eff ect of polyphenols on DPPH· was higher than Vc in rang of 0.1-1.6 μg/mL. When the concentration is greater than 0.6 μg/mL, the scavenging eff ects of plyphenols on ·OH is higher than Vc. The total reducing ability of polyphenols from PGP improved with the increase of concentration, and when the concentration was 8 μg/mL, the total reducing ability was 114 U/mL.

Key words: pomeranate peel, total polyphenols, extraction process, orthogonal experiment, antioxidant activity