多酚杂志

本期目录

2019年, 第1卷, 第1期　刊出日期：2019-04-16   

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Simultaneous determination of gallic acid and p-coumaric acid in rat plasma by UPLC-MS/MS and its application to a comparative pharmacokinetic study after oral administration of monomer compound and red wine extract Huijie Lv, Mengyu Zou, Weichao Yu, Baoshan Sun, Yan Cui 2019 (1):  1-11.  摘要 ( 1206 )   PDF(4626KB) ( 586 )   Abstract A rapid, sensitive and selective ultra high performance liquid chromatography-tandem mass spectrometry (UPLCMS/MS) method was developed and validated for simultaneous determination of gallic acid (GA) and p-coumaric acid (CA) in rat plasma. Plasma samples were extracted by methanol and separated on an ACQUITY UPLC BEH C18 column (1.7 μm, 100 mm × 2.1 mm) using gradient elution consisting of acetonitrile – 0.2% formic acid within a runtime of 4.0 min. The detection was performed in multiple reaction monitoring (MRM) mode with negative ionization. The linear range was 20– 20000 ng/mL for both GA and CA, with lower limits of quantification of 20 ng/mL. Intra-day and inter-day precisions were within 5.4% and 10.0%, respectively and the accuracy (relative error, RE, %) was less than 7.2% and –4.9%, respectively. The mean absolute extraction recoveries of both analytes and IS from rat plasma were all more than 82.6%. The validated method was successfully applied to the comparative pharmacokinetic study of GA and CA in rat plasma after oral administration of GA and CA monomers and red wine extract, respectively. It was found that both the area under the curve (AUC) and t1/2 of the two constituents were remarkably increased for red wine extract group than that in monomer group, indicating the priority of intake of red wine to active component monomer 相关文章 | 计量指标

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Improvement of Vitis amurensis Rupr. grape quality by using fish protein hydrolysates as fertilizer Jing Li, Yingjie Dong, Wei Zhou, Haiyan Wang, Xiaochun Huang, Shuting Zhang, Wei Zheng, Baoshan Sun 2019 (1):  12-22.  摘要 ( 941 )   PDF(39218KB) ( 225 )   High quality wine grape is the first factor to satisfy the wine-maker to produce superior quality wines. In this work, a fish protein hydrolysates derived from deep-sea salmon or cod, was used as fertilizer to improve Shuanghong (Vitis amurensis Rupr.) grape quality. Application of powder fish protein hydrolysates fertilizer by spraying directly to foliar (4.5 kg/ha), to root (4.5 kg/ha) or to the both foliar (4.5 kg/ha) and root (4.5 kg/ha) were carried out respectively, during the period from 2–3 weeks before flowering until 2–3 weeks before harvest. Grape sampling was made at both veraison and harvest. Different classes of phenolic compounds, including 10 phenolic acids and 11 proanthocyanidins in grape seeds and 10 anthocyanins in grape skins were determined by UHPLC or HPLC; total anthocyanins and total polyphenols in grape skins were determined by spectrophotometric method. Antioxidant activities of the phenolic compounds were evaluated by ABTS and FRAP methods. The results showed that the application of fish protein hydrolysates fertilizer to both root and foliar led to a significant improvement of berry quality at harvest/maturity, i.e., increasing 12.13% total polyphenols (TP), 8.3% total anthocyanins 11.03% ABTS and 45.81% FRAP compared with control. Besides, both root and foliar application resulted in the highest individual anthocyanins and proanthocyanidins in grapes. These results suggest that the both root and foliar application could be of great interest for the viticulturists to produce grapes with higher polyphenolic content and antioxidant activity. Although this work was experimented with Vitis amurensis Rupr. grapes, the proposed methods would also be applicable to other grape varieties for the purposes of increasing anthocyanin or polyphenol contents. 相关文章 | 计量指标

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The fingerprint and flavonoids contents by HPLC and the UPLC-DAD-Q-TOF-MS analysis of Sedum aizoon L. Tanye Xu, Chongning Lv, Jincai Lu 2019 (1):  23-32.  摘要 ( 770 )   PDF(3270KB) ( 668 )   Fingerprint analysis of Sedum aizoon L. was carried by High-performance liquid chromatography. There were 27 common peaks in the fingerprint of 10 samples. Eight of these common peaks, peaks 1 (arbutin), 4 (gallic acid), 10 (myricetin- 3-O-β-D-glucopyranoside), 14 (myricetin-3-O-α-L-rhamnopyranoside), 19 (quercetin-3-O-α-L-rhamnopyranoside), 22 (kaempferol-3-O-α-L-rhamnopyranoside), 24 (quercetin) and 26 (kaempferol) were identified by reference substances. Moreover, six flavonoid compounds were simultaneously determined by the above method. By comparing with the constituents in the aerial parts, roots and flowers, it’s easy found that the aerial parts of S. aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots, while the flavonoid glycosides largely existing in the flowers. In addition, an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S. aizoon. This study may provide a preliminary reference for the quality control of S. aizoon. 相关文章 | 计量指标

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Variation in phenolic composition and color features of red wine during vinification Chen Wang, Lingxi Li, Yuanyuan Li, Baoshan Sun, Shuting Zhang 2019 (1):  33-49.  摘要 ( 1041 )   PDF(1355KB) ( 892 )   The compositions and contents of phenolic components during the three periods (alcoholic fermentation, malolactic fermentation and aging process) of wine making by Cabernet Sauvignon were analyzed by high performance liquid chromatography. The color parameters of the wine were also determined by CIELab method. Correlation analysis and principal component analysis were adopted to analyze the correlation between phenolic content and color parameters. In fermentation and aging periods, anthocyanins and other phenolic components in wine increased at first, and before the end of malolactic fermentation, the contents reached the highest, and later experienced a downward trend, then contents change showed a stable trend in the later stage of storage. During the whole wine-making, anthocyanins had significant correlation with CIELab parameter a* and anthocyanin malvidin-3-O-glucoside was mainly observed to present a large impact on a* in aging period, causing the red color change of wine. Other phenolic components affected the CIELab parameters a*, b* and L* differently. Statistical analysis about the color parameters and 15 phenolic components revealed correlations between the color properties and detectable phenolic contents. 相关文章 | 计量指标

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Cranberry cultivated in China: UPLC-Q-TOF-MS analysis of its acidic ethanol extract and assessment of its anti-bacterial and anti-tumor activities in vitro Changhua Li, Lin Wang, Dianwen Wei, Gaosheng Hu, Xiaoning Zhang, Zhihong Lou, Qiong Zhang, Jingming Jia, Yongsheng Hou 2019 (1):  50-61.  摘要 ( 939 )   PDF(3753KB) ( 819 )   Cranberry, the fruit of Vaccinium macrocarpon Aiton, is becoming popular all over the world, due to its pleasant taste, nutraceutical value and biological activities. For a long time, 98% of its yield has been from America. Since being introduced to China as an import in recent decades, cranberry has become a rapid success in the food industry even with the high transportation cost. Recently, V. macrocarpon was cultivated in the Northeast of China, and the area of cultivation has increased dramatically. Therefore, it is important to systematically determine the useful compounds and their activity in this “Made-in-China” cranberry. In the present study, the content of total phenolics, anthocyanins, proanthocyanidins, sugars and organic acids was determined. Our results demonstrated that domestic cranberry is a rich source of phenolics, anthocyanins, proanthocyanidins, sugars and organic acids. Analysis of the chemical composition of an acidic ethanol extract by UPLC-QTOF-MS identified 21 compounds as well as the contents determination of catechin and cyanidin-3-O-glucoside using RPHPLC. Furthermore, cranberry extract was tested for its anti-bacterial activity against five clinically important pathogens, as well as its inhibitory effect on the proliferation and migration of cancer cells in vitro. Our results demonstrated that cranberry produced in Chinese cultivation areas is a rich source of bioactive compounds and exhibited in vitro anti-microbial and anti-tumor activities. Our work provides essential information about the quality of domestic cranberry and facilitates the development of related industries. 相关文章 | 计量指标

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In silico study of wine anthocyanins and their polymeric pigments on human pancreatic α-amylase and salivary α-amylase Lingxi Li, Shuting Zhang, Baoshan Sun 2019 (1):  62-69.  摘要 ( 896 )   PDF(588KB) ( 616 )   Polyphenols, such as procyanidins and anthocyanins, as natural α-amylase inhibitors, have been extremely studied about their inhibitory activity in vitro. In this work, molecular docking was used to explore the efficacy of wine anthocyanins and their polymeric pigments to inhibit human pancreatic α-amylase (HPA) and salivary α-amylase (HSA). Three residues, ASP197, GLU233 and ASP300 were proposed as main interacting residues with both HPA and HSA. Hydrogen bonds, π-π stacking, hydrophobic interaction and electrostatic interaction played important roles in binding. Polymeric pigments showed stronger affinity with HPA and might be a potential α-amylase inhibitor. Moreover, according to the docking result of HSA, polymeric pigments exhibited more impact on astringency than anthocyanins. 相关文章 | 计量指标

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Phenolic compounds obtained from the fruit of Crataegus pinnatifida with neuroprotective activities Rui Guo, Fengying Han, Tianming Lv, Xiaoxiao Huang, Shaojiang Song 2019 (1):  70-75.  摘要 ( 836 )   PDF(6006KB) ( 611 )   Ten phenolic compounds (1a/1b, 2–9) including a pair of enantiomers (1a/1b), along with eight analogues (2–9) were isolated from the fruit of Crataegus pinnatifida. Enantiomers 1a/1b were separated successfully by chiral chromatographic column. Their structures were established by comprehensive spectroscopic analyses, and the absolute configurations of enantiomers were determined by comparison between the experimental and calculated electronic circular dichroism (ECD) spectra. In addition, all isolates were investigated for their neuroprotective effects against H2O2 – induced oxidative stress in human neuroblastoma SH-SY5Y cells. It was found that enantiomers 1a and 1b displayed significant neuroprotective activities but no enantioselectivity. In addition, compounds 3–6 showed obvious neuroprotective effects at different concentrations, while compound 8 exhibited potential neuroprotective effect at higher concentration (50 μmol/L). 相关文章 | 计量指标