多酚杂志

本期目录

2022年, 第4卷, 第1期　刊出日期：2022-09-26 上一期   

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RNA-seq analysis of protective effect of epicatechin gallate on H2O2-induced oxidative injury in PC12 cells Nan Cheng, Xinxin Lin, Jiayue Xu, Kai Wang, Kaile Wang, Zhengping Liu, Parhat Kamel, Fang Wang 2022 (1):  1-16.  摘要 ( 552 )   PDF(35754KB) ( 100 )   Abstract Epicatechin gallate (ECG) is one of the polyphenolic compounds and has attracted much attention due to its various bioactivities. In this study, the neuroprotective effect of ECG against H2O2-induced oxidative injury in PC12 cells as well as the possible mechanisms were investigated. Cell viability was determined by MTT assay. The differentially expressed genes (DEGs), GO enrichment, and KEGG enrichment were analyzed to explore the mechanism of ECG against H2O2-induced oxidative injury by using the RNA-seq method. Finally, the change in the cell cycle was analyzed by flow cytometry. H2O2 (400-1200 μ mol/L) inhibited the cell viability in a concentration-dependent manner. ECG (6-150 μmol/L) eff ectively attenuated the H2O2-induced decrease in cell viability. RNA-seq analysis showed that ECG regulated 1 058 coexpressed DEGs. GO enrichment analysis showed that the cellular component was the dominant group after ECG treatment. KEGG analysis showed that the cell cycle, fanconi anemia pathway, and homologous recombination were the important pathways for ECG in improving H2O2-induced oxidative injury and 28 coexpressed DEGs in the cell cycle pathway were summarized. Finally, cell cycle analysis also proved that ECG improved H2O2-induced cell cycle arrest in the G2/M phase. Our present study demonstrated that ECG attenuated H2O2-induced neurologic oxidative damage by multiple modulatory mechanisms at the molecular transcription level. These fi ndings provide new insights for further study of the molecular mechanism of the neuroprotection of ECG. 相关文章 | 计量指标

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Noradrenergic, glutamatergic and dopaminergic systems are implicated in antidepressant-like effect of flavonoids extracted from okra (Abelmoschus esculentus [L.] Moench.) fruit in the forced swimming test Fuyuan Li, Xiaozhuo Zhang, Qianqian Mao, Bo Wu, Ying Jia, Tingxu Yan 2022 (1):  17-31.  摘要 ( 484 )   PDF(1006KB) ( 319 )   Abstract Okra (Abelmoschus esculentus [L.] Moench.) is one of the most frequently used herbals in East or West Africa, and its various biological activities have been widely studied. Flavonoids extracted from many plants are reported to have neurological properties, e. g antidepressant and antifatigue. However, its neurological protect in antidepressant-like effect of fl avonoids extracted from okra have not yet been demonstrated. The present study was aimed at investigating the antidepressant-like effect of the fl avonoids extracted from okra fruit (FOF) using the forced swimming test (FST) pattern and preliminary exploration its potential mechanism. We also used the open field test (OFT) to estimate the spontaneous locomotor activity. We found that oral administration (p. o.) of FOF (300 mg/kg) alone signifi cantly reduced the immobility time in the FST without changes in locomotor activity in the OPT. The experimental data indicated the antidepressant-like effect of FOF involved in noradrenergic, glutamatergic and dopaminergic systems. 相关文章 | 计量指标

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Efficient semisynthetic approach for large preparation of procyanidin C1 through degradation of grape seed polymeric procyanidins Qian Li, Lina Zhang, Danyang Wang, Mengyao Zhao, Baoshan Sun, Shuting Zhang 2022 (1):  32-41.  摘要 ( 555 )   PDF(2593KB) ( 261 )   Abstract An efficient method for producing trimeric procyanidin C1 (PCC1) was developed through degradation of grape seed polymeric procyanidins (PPCs), using epicatechin (EC) as nucleophile and hydrochloric acid as catalyst. With the yield of PCC1 as the evaluation index, the degradation conditions were optimized by Box-Behnken Design (BBD) based on the results of single-factor experiments. The results showed that the optimal conditions were reaction temperature of 40 ℃, the ratio of EC and PPCs 2:1, acidity of 0.10 mol/L, and reaction time of 20 min. The yield of PCC1 reached up to 17.7 mg by only one-step degradation of 3 g PPCs. This work proposed a new method for large preparation of PCC1 from waste grape seed polymeric procyanidins. 相关文章 | 计量指标

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Pharmacological effects and mechanisms of polyphenols in Radix Puerariae on liver protection and anti-diabetes Yerong Yuan, Zihui Wei, Zunchao Liu, Wei Li, Jiaqing Cao, Xiangrong Zhang 2022 (1):  42-54.  摘要 ( 598 )   PDF(1035KB) ( 665 )   Abstract In recent years, the incidence of liver diseases and diabetes is increasing year by year, and the patients are younger than before. Although the clinical medicine treatment is effective, but it would produce side eff ects. Radix Puerariae is the dried root of the Pueraria lobata (Willd.) Ohwi. Puerarin is an isofl avone compound with polyphenol structure. It is one of the main bioactive components of Radix Puerariae. Studies have found that the application of puerarin offers beatifical act on liver protection, hypoglycemia, blood lipid, antioxidant, anti-osteoporosis, anti-cancer, anti-inflammatory and cardiovascular protection. Puerarin has been used in the treatment of liver disease and diabetes. In this study, the effect of puerarin on liver protection, diabetes treatment and the relationship between the two diseases were reviewed. The therapeutic mechanism and molecular targets of puerarin were explored in order to further improve the development of puerarin and increase its clinical application in protection against liver disease and diabetes. 相关文章 | 计量指标

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Galangin probably ameliorates hyperuricemia by inhibiting urate acid transport 1 (URAT1): Homology modeling and mechanism exploration Aijinxiu Ma, Fuqi Wang, Xu Zhao 2022 (1):  55-63.  摘要 ( 547 )   PDF(2075KB) ( 295 )   Abstract Urate acid transporter 1 (URAT1) is the main transporter of uric acid reabsorption, which closely related to the pathogenesis of hyperuricemia. Screening URAT1 inhibitors and studying their possible metabolic processes is a hot spot in the development of uric acid-lowering drugs. Studies have shown that many food-borne plant polyphenols have uric acid lowering activity with non-toxic side eff ects, and can be used to improve and alleviate hyperuricemia. In this study, we take galangin (GAL) as an example to explore the mechanism of plant polyphenols affecting hyperuricemia by inhibiting URAT1. Homology modeling was used to construct a three-dimensional model of URAT1 protein, and the structure was optimized. Ramachandran diagram was used to verify the rationality of model protein structure. A known URAT1 inhibitor, benzbromarone (BBR), was used to dock with URAT1 to determine the docking site and show the key amino acids. GAL and model protein were docked by molecular docking method to analyze their interaction. Meanwhile, comparing the interaction of BBR and GAL with the key amino acids of model proteins, the binding of GAL was more stable, suggesting that GAL could aff ects hyperuricemia by inhibiting URAT1. This paper aims to provide theoretical guidance for the development of new functional food ingredients for lowering uric acid. 相关文章 | 计量指标