Journal Of
Polyphenols

Abstract: Fingerprint analysis of Sedum aizoon L. was carried by High-performance liquid chromatography. There were 27 common peaks in the fingerprint of 10 samples. Eight of these common peaks, peaks 1 (arbutin), 4 (gallic acid), 10 (myricetin- 3-O-β-D-glucopyranoside), 14 (myricetin-3-O-α-L-rhamnopyranoside), 19 (quercetin-3-O-α-L-rhamnopyranoside), 22 (kaempferol-3-O-α-L-rhamnopyranoside), 24 (quercetin) and 26 (kaempferol) were identified by reference substances. Moreover, six flavonoid compounds were simultaneously determined by the above method. By comparing with the constituents in the aerial parts, roots and flowers, it’s easy found that the aerial parts of S. aizoon had abundant flavonoids and the flavonoid aglycones mostly exist in the roots, while the flavonoid glycosides largely existing in the flowers. In addition, an UPLC-DAD-Q-TOF-MS method was developed for qualitative analysis of the aerial parts of S. aizoon. This study may provide a preliminary reference for the quality control of S. aizoon.

Key words: Sedum aizoon L., HPLC fingerprint, flavonoids, UPLC-DAD-Q-TOF-MS